SNAP-8
Acétyl Octapeptide-3 (Acetyl Glutamyl Octapeptide)
SNAP-8 (Acetyl Octapeptide-3) is a cosmetic octapeptide derived from Argireline (Acetyl Hexapeptide-8), extended by two amino acids for a reinforced action on the SNARE complex. Applied exclusively topically (serum or cream), it modulates neurotransmitter release at the facial neuromuscular junction, which reduces the micro-contractions responsible for expression lines. A cosmetic, non-invasive alternative to filler or injection protocols.
Chemical identity
- Sequence
- Ac-Glu-Glu-Met-Gln-Arg-Arg-Ala-Asp-NH2 (Acétyl Octapeptide-3)
- Molecular formula
- C42H72N16O15S
- Molecular weight
- 1073.2 g/mol
- CAS no.
- 868844-74-0
Purity (HPLC) measures the absence of related impurities; it is distinct from net peptide content, since salts and counter-ions (acetate, TFA) count toward the vial mass. Account for this when computing the real concentration at reconstitution.
Potential benefits
Smoothing of expression lines
Visually reduces forehead lines, crow's feet, and glabellar lines linked to repeated micro muscle contractions.
Non-invasive alternative
Reproduces a "botox-like" effect topically, with no needle or medical procedure.
Targeted, local action
Acts at the level of the skin's neuromuscular junction, with no systemic aim.
Fits a daily routine
Easily incorporated into a serum or cream, for twice-daily use.
Cosmetic synergy
Combines with other anti-aging actives (peptides, antioxidants, moisturizers) for an overall effect.
Favorable skin tolerance
Generally good tolerance profile, with irritation risk limited at usual concentrations (3 to 10%).
Mechanism of action
- SNAP-8 mimics the N-terminal portion of SNAP-25, a key protein of the SNARE complex involved in synaptic vesicle fusion.
- By occupying this site, it competes with native SNAP-25 and slows the full assembly of the SNARE complex.
- This interference reduces acetylcholine release at the facial neuromuscular junction.
- The underlying muscle fibers contract with less intensity, which limits repeated surface creasing of the skin.
- Unlike botulinum toxin, the action is competitive and reversible, with no irreversible protein cleavage of the SNARE proteins.
Historical milestones
Research milestones, clinical trials and regulatory steps.
- 2002
Argireline predecessor
Lipotec (Barcelona) develops Argireline (Acetyl Hexapeptide-3), the first cosmetic peptide targeting the SNARE complex, of which SNAP-8 is the evolution.
- 2006
Development of SNAP-8
Lipotec develops SNAP-8 (Acetyl Octapeptide-3), an elongated eight-amino-acid analog mimicking the N-terminal end of the SNAP-25 protein.
- 2006
Anti-wrinkle mechanism
By competing with SNAP-25 in the SNARE complex, the peptide aims to reduce neurotransmitter release and soften expression lines.
- 2010s
Acquisition by Lubrizol
Lipotec comes under Lubrizol Life Science, which continues to market SNAP-8 as a topical cosmetic active.
- 2020s
Cosmetic status
Classified as a cosmetic ingredient (not a drug) and not FDA-regulated, SNAP-8 is widely used in anti-aging skincare at 3-10% concentrations.
Evolution over time
Application and tolerance; skin feels more supple, first hydrating effects from the serum.
Progressive reduction of fine expression lines.
More pronounced smoothing on the forehead and eye contour with regular twice-daily application; reported results vary depending on concentration and skin type.
Maintenance of results subject to continued application; discontinuation leads to a gradual return to baseline.
Dosages & protocol
Reference dosages
12 wk + 4 off
Daily application, morning and evening, on clean, dry skin
Not applicable for topical use: a cosmetic peptide acting locally on the skin with no significant systemic exposure; the concept of plasma half-life does not apply.
Application
A topical product — no reconstitution or injection required. Follow the supplier's instructions for the serum or cream formulation.
Serum preparation and application
SNAP-8 is supplied as a freeze-dried powder for topical use only: it is never injected.
- The freeze-dried powder (10 mg) dissolves in an aqueous base or a neutral serum to obtain a concentrated stock solution.
- This stock solution is then incorporated at 3 to 10% into a final cream or serum, depending on the desired concentration.
- Never inject: SNAP-8 is formulated exclusively for external cutaneous application.
- Gently swirl the vial during reconstitution, without shaking the solution vigorously.
- Store the reconstituted preparation in the refrigerator and use within a limited timeframe to preserve peptide stability.
What it is not
- It is not an injection or a substitute for botulinum toxin.
- It is not an immediate-effect product; results build up gradually over several weeks.
- It is not a medical treatment; it is a cosmetic active intended for research and aesthetic use.
- It is not indicated on damaged, irritated skin, or in case of known allergy to any of the components.
Who is SNAP-8 for?
Suited to anyone seeking a cosmetic routine targeting expression lines, without resorting to an injectable procedure.
- People with forehead lines, crow's feet, or glabellar lines linked to repeated facial expressions.
- People looking for a gentle, non-invasive alternative to injections.
- People wishing to add a targeted peptide active to an existing anti-aging routine.
- People mindful of skin tolerance, with a gradual increase in concentrations.
Usage tips
- Perform a skin patch test 24-48h before any broader application, especially at 10%.
- Apply to clean, dry skin, morning and/or evening, avoiding direct contact with the eyes.
- Start at a low concentration (3%) then increase gradually according to tolerance.
- Combine with daily sun protection to preserve anti-aging benefits.
- Store the freeze-dried powder in the refrigerator (2-8°C), protected from light, before reconstitution.
Good to know / effects to watch
- Possible irritation, redness, or tightness, especially at higher concentrations (10%).
- Increased risk on reactive, eczematous, or damaged skin.
- Rare but possible skin sensitization with prolonged use without a break.
- Transient discomfort if applied too close to the eyes or mucous membranes.
- Possible skin interactions if combined, untested, with exfoliating or irritating actives.
- Reversible cosmetic effect: no guarantee of results identical to an injectable medical procedure.
Storage
- Lyophilized powder: refrigerate (2-8 °C), away from light and moisture.
- Serum/cream after incorporation: keep refrigerated, protected from light and air.
- Use the reconstituted serum within a limited time (a few weeks); discard if color, appearance or odor changes.
Contraindications
- Broken, irritated, eczematous skin or active dermatosis.
- Known allergy or hypersensitivity to the peptide or a formula component.
- Application on or near the eyes and mucous membranes.
- Pregnancy and breastfeeding: as a precaution, seek professional advice before use.
Possible synergistic combinations
The catalog peptides are clickable — to complete or optimize your protocol.
Peptide from the same family; association sometimes used to reinforce the overall smoothing effect.
Provides complementary hydration and improves the serum's texture and penetration.
Frequent cosmetic pairing to target both expression lines and skin regeneration.
View the sheet & add →Sources & references
Links to external sources (scientific databases, trial registries, authorities). RAL Peptides is not responsible for their content.
⚠ For research use only. NOT intended for use on humans or animals. The values shown are indicative and for informational purposes ; each person reacts differently. This guide does not replace medical advice — consult a healthcare professional if in doubt.